The reagents used in this study are as follows: Drugs Honokiol (MCE,CAT#HY-N0003, purity is 99.90%), ATP (MCE,CAT#HY-B0345A/CS-2387), ASC oligomerization inhibitor MCC950 (MCE,CAT#HY-12815A), Caspase-1 inhibitor VX-765 (MCE,CAT#HY-13205), LPS (Sigma,CAT#L2880), collagenase IV (BioFroxx,CAT#2091MG100), DNase I (SAITONG,CAT#PS0825), single-cell suspensions kit (Solarbio,CAT#P5380), RPMI-1640 media (Gibco, REF:C11875500BT), FBS (Multicell, CAT NO:086-150), p3*flag-NLRP3 (PPL, CAT#PPL00151-2b), P3 Primary Cell 4D-Nucleofector X Kit L (Lonza,CAT#V4XP-3024), Bradford Assay kit(Beyotime,CAT#P0006), Blood Urea Nitrogen (BUN) Detection Kit (Nanjing Jiancheng Bioengineering Institute,CAT#C013-2-1), Creatinine Assay Kit (Nanjing Jiancheng Bioengineering Institute,CAT#C011-2-1), IL-33 (Affinity,CAT#DF8319),NLRP3 (Affinity, CAT#DF74), PrimeScript RT Reagent Kit (Takara, CAT#RR037A), HieffTM qPCR SYBR Green Master Mix (YEASEN, CAT#11201ES08), RIPA lysis buffer (Beyotime,CAT#P0013B), Nuclear protein Extraction Kit (Solarbio,CAT#R0050), BCA Protein Assay kit (Beyotime, CAT#P0012), PVDF membranes (Millipore,CAT#ISEQ00010), NLRP3 (CST,CAT#20836T), Cleaved caspase-1 (Affinity,CAT#AF4005), Cleaced IL-1β(CST,CAT#20839T), Caspase-1 (CST,CAT#20842T), ASC (CST,CAT#20838T), IL-33 (Affinity,CAT#DF8319), ST2 (proteintech,CAT#60112-1-Ig), NF-κB (Affinity,CAT#BF8005), Tublinβ (Affinity,CAT#AF7011), Lamin B (Affinity,CAT#BF8009), GAPDH (Abbkine,CAT#KTD101-CN), secondary rabbit-antibody (Immunoway, CAT#RS23920), secondary mouse-antibody (Abbkine,CAT#A23710), Mouse IL-33 ELISA Kit (MultiSciences,CAT#70-EK233/2-48), Mouse IL-1β ELISA Kit (FANKEWEI,CAT#F2923-B), Rat IL-1β ELISA Kit (FANKEWEI,CAT#F2923-B), Rat IL-18 ELISA Kit (FANKEWEI,CAT#F3070-B). Recombinanl NLR Family, Pyrin Domain Containing Protein 3(NLRP3) (Cloud-Clone, CAT#RPK115Hu01).
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