LM pectin (GENU pectin LM-102AS-J) was provided
by Sansho Co. Ltd. (Osaka, Japan). The average degree of methoxylation
was ∼30% and that of amidation was ∼19%. CaCO3 and GDL were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka,
Japan). Bromothymol blue (BTB) solution (0.04%) was purchased from
Kanto Chemical Co. Inc. (Tokyo, Japan). SodaStream Spirit One Touch
(SodaStream International Ltd., Israel) was employed to produce the
carbonated water. The water used in this study was purified using
a Milli-Q system (Nihon Millipore Co., Tokyo, Japan). All reagents
were used without further purification. Dulbecco’s modified
Eagle medium (DMEM), penicillin–streptomycin (PS), and fetal
bovine serum (FBS) were purchased from Life Technologies Corp. (Grand
Island, NY, USA). Cell Counting Kit-8, Calcein-AM, and Ethidium homodimer
(EthD-1) were purchased from DOJINDO Lab. Co. (Kumamoto, Japan).
by Sansho Co. Ltd. (Osaka, Japan). The average degree of methoxylation
was ∼30% and that of amidation was ∼19%. CaCO3 and GDL were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka,
Japan). Bromothymol blue (BTB) solution (0.04%) was purchased from
Kanto Chemical Co. Inc. (Tokyo, Japan). SodaStream Spirit One Touch
(SodaStream International Ltd., Israel) was employed to produce the
carbonated water. The water used in this study was purified using
a Milli-Q system (Nihon Millipore Co., Tokyo, Japan). All reagents
were used without further purification. Dulbecco’s modified
Eagle medium (DMEM), penicillin–streptomycin (PS), and fetal
bovine serum (FBS) were purchased from Life Technologies Corp. (Grand
Island, NY, USA). Cell Counting Kit-8, Calcein-AM, and Ethidium homodimer
(EthD-1) were purchased from DOJINDO Lab. Co. (Kumamoto, Japan).
Free full text: Click here
