A specific SW1990 cell line (SW1990-LNC-KD) with the stable knock down of lncRNA ELFN1-AS1 and a scramble control SW1990 cell line were constructed with lnc-small hairpin RNA (shRNA) or a scramble control (see Table S1 ) cloned into the pLV3 (H1/GFP & Puro) vector (see Figure S1 ) and transfected into 293T cells (Shanghai GenePharma China). Supernatants were collected after 72 h incubation and used for the transfection (24 h incubation before transfection) of the SW1990s (12 (link)). SW1990 cells containing the lnc-shRNA were screened by media containing 5 µg/mL puromycin (Sigma, St. Louis, MO, USA), and used for the Xenograft tumor growth experiments (13 (link)).
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Stable Knockdown of lncRNA ELFN1-AS1 in SW1990 Cells
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293t cells
Cell line
Cells
Experiments tumor
Line
Lncrna
Puromycin
Shrna
Transfection
Vector
Xenograft
Corresponding Organization :
Other organizations : First Hospital of China Medical University, China Medical University
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Variable analysis
independent variables
- Stable knockdown of lncRNA ELFN1-AS1 in SW1990 cell line (SW1990-LNC-KD)
- Scramble control SW1990 cell line
- Xenograft tumor growth
- Scramble control SW1990 cell line
- Puromycin (5 µg/mL) for selecting SW1990 cells containing the lnc-shRNA
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