Isolation and Preservation of Leukocytes

Leukocyte was isolated according to the method of O’Loughlin et al [12 (link)]. Briefly, blood leukocytes were isolated using a hypotonic solution and a hypertonic solution. The leukocyte pellets were suspended in 1 mL of TRI Reagent® solution (TRIzol) (Sigma-Aldrich Ireland Ltd., Dublin, Ireland), and stored in a sterile tube at −70°C until RNA isolation.

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Yoo S., Beak S.H., Kang H.J., Jung D.J., Fassah D.M., Jeong I., Park S.J., Haque M.N., Kim M, & Baik M. (2023). Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves. Animal Bioscience, 36(3), 521-528.

Publication 2023

Hypertonic solution Hypotonic solution Isolation Leukocyte Pellets Sterile Trizol

Corresponding Organization :

Other organizations : Seoul National University, Pusan National University

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Variable analysis

independent variables

Isolation method described by O'Loughlin et al. [12], which involves using a hypotonic solution and a hypertonic solution to isolate blood leukocytes

dependent variables

RNA isolated from the leukocyte pellets

control variables

Leukocyte pellets were suspended in 1 mL of TRI Reagent® solution (TRIzol) and stored in a sterile tube at −70°C until RNA isolation

controls

No positive or negative controls were explicitly mentioned in the provided information.

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