2D Gel Electrophoresis Protein Profiling

Protein extracts were separated by two-dimensional gel electrophoresis as previously described [53 (link)]. Gels were then labeled with Flamingo Fluorescent Gel Stain (Bio-Rad, Hercules, CA, USA), scanned in a Typhoon 9400 (GE Healthcare, Chicago, IL, USA), and analyzed for spot variations using PD-Quest 8.0 (Bio-Rad, Hercules, CA, USA). Three animals from each group were analyzed. Spots of interest were then excised and identified by matrix-assisted laser desorption/ionization-time-of-flight using an AutoFlex III Smartbeam MALDI-ToF/ToF (Bruker Daltonics, Billerica, MA, USA).

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Gallinat A., Vilahur G., Padró T, & Badimon L. (2022). Network-Assisted Systems Biology Analysis of the Mitochondrial Proteome in a Pre-Clinical Model of Ischemia, Revascularization and Post-Conditioning. International Journal of Molecular Sciences, 23(4), 2087.

Publication 2022

Flamingo Fluorescent Gel Stain Typhoon 9400 PD-Quest 8.0 AutoFlex III Smartbeam MALDI-ToF/ToF

Animals Maldi Protein Spots Stain Two dimensional gel electrophoresis Typhoon

Corresponding Organization : Instituto de Salud Carlos III

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Variable analysis

independent variables

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dependent variables

Protein spot variations

control variables

Three animals from each group were analyzed

controls

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