The tumor cell lines tested negative for mycoplasma infection and Plasmocin (InvivoGen) was used as a routine addition to the culture media to prevent mycoplasma contamination. The mice were inoculated with 1–1.5 × 105 B16F10-OVA cells or 2 × 106 MC38-OVA cells s.c. into the right flank. The mice were i.p. injected at the indicated time points with either 200 μg anti-PD-1 (29F1. A12, InVivoPlus anti-mouse PD-1, Bioxcell), anti-CTLA-4 (9H10, InVivoPlus anti-mouse CTLA-4, Bioxcell) or the respective isotype controls (anti-CTLA-4 isotype control, InVivoPlus polyclonal Syrian hamster IgG, Bioxcell; anti-PD-1 isotype control, InVivoPlus rat IgG2a isotype control, anti-trinitrophenol, Bioxcell). Tumor size was monitored every other day and tumors were harvested at the indicated time points for the analysis of tumor-infiltrating lymphocytes. Tumor volume was calculated as ½ × D × d2, where D is the major axis and d is the minor axis, as described previously45 (link). Tumor growth was monitored at least three times a week to ensure that the tumors did not exceed 25 mm in diameter.
Protocol detail
Tumor Growth Inhibition by Immune Checkpoint Blockade
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Axis
Cells
Ctla 4
Culture media
Igg2a
Isotype
Mouse
Mycoplasma
Mycoplasma infection
Plasmocin
Syrian hamster
Trinitrophenol
Tumor cell lines
Tumor infiltrating lymphocytes
Tumors
Corresponding Organization :
Other organizations : La Jolla Institute For Allergy & Immunology, NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust, NIHR CRUK Experimental Cancer Medicine Centre, University of Southampton, University of Nottingham, University of California, San Diego
Top 5 similar protocols
Variable analysis
independent variables
- Anti-PD-1 antibody treatment
- Anti-CTLA-4 antibody treatment
- Respective isotype controls
- Tumor size
- Tumor-infiltrating lymphocytes
- Tumor cell lines tested negative for mycoplasma infection
- Plasmocin (InvivoGen) was used as a routine addition to the culture media to prevent mycoplasma contamination
- Mice were inoculated with 1–1.5 × 10^5 B16F10-OVA cells or 2 × 10^6 MC38-OVA cells s.c. into the right flank
- Tumor size was monitored to ensure that the tumors did not exceed 25 mm in diameter
- Anti-CTLA-4 isotype control, InVivoPlus polyclonal Syrian hamster IgG, Bioxcell
- Anti-PD-1 isotype control, InVivoPlus rat IgG2a isotype control, anti-trinitrophenol, Bioxcell
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!