AB strain zebrafish were raised and maintained under standard laboratory conditions at 28.5°C [17 (link)]. For whole mount in situ hybridization experiments, embryos were raised in 0.2 mM 1-phenyl-2-thiourea (Sigma) to block pigmentation.
The transgenic fish Tg:[(fabp10a:dsRed;ela3A:GFP)gz15] line was generated by the Gong laboratory [18 (link)] and shared with us by the Leach laboratory. The transgenic fish Tg:[(P0-pax6b:GFP)ulg515] (abbreviated pax6b:GFP) was generated by the Martial laboratory [19 (link)] and shared with us by the Chen laboratory. For live imaging WT and sar1b-MO larvae were anesthetized in Tricaine (Sigma) and mounted in low-melt agarose. All confocal imaging in this manuscript was taken with a Zeiss LSM510 inverted confocal microscope (Vanderbilt Cell Imaging Shared Resource), while all other imaging was taken with a Zeiss AxioImager Z1. All experiments were conducted in accordance with the guidelines established by the IACUC at Vanderbilt University.
The transgenic fish Tg:[(fabp10a:dsRed;ela3A:GFP)gz15] line was generated by the Gong laboratory [18 (link)] and shared with us by the Leach laboratory. The transgenic fish Tg:[(P0-pax6b:GFP)ulg515] (abbreviated pax6b:GFP) was generated by the Martial laboratory [19 (link)] and shared with us by the Chen laboratory. For live imaging WT and sar1b-MO larvae were anesthetized in Tricaine (Sigma) and mounted in low-melt agarose. All confocal imaging in this manuscript was taken with a Zeiss LSM510 inverted confocal microscope (Vanderbilt Cell Imaging Shared Resource), while all other imaging was taken with a Zeiss AxioImager Z1. All experiments were conducted in accordance with the guidelines established by the IACUC at Vanderbilt University.
