Bone marrow cells were analyzed for retention of biotin labeling as previously described [26 (link)]. Cell surface markers used for analysis were identical to those used for cell cycle analysis with the following exceptions: biotinylated Ryk was replaced with sheep anti-mouse Ryk (AF4649, R&D Systems) with Alexa-Fluor 546-conugated donkey anti-sheep IgG acting as a secondary antibody (Invitrogen, Carlsbad, CA, http://www.invitrogen.com ). APC-conjugated streptavidin (eBioscience) was used to detect biotin-labeled cells.
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Bone Marrow Cell Biotin Retention Analysis
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Alexa fluor 546
Anti igg
Antibody
Biotin
Bone marrow cells
Cell cycle
Cells
Donkey
Mouse
Retention
Sheep
Streptavidin
Corresponding Organization :
Other organizations : Roswell Park Comprehensive Cancer Center
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independent variables
- Biotinylated Ryk was replaced with sheep anti-mouse Ryk (AF4649, R&D Systems) with Alexa-Fluor 546-conugated donkey anti-sheep IgG acting as a secondary antibody (Invitrogen, Carlsbad, CA, http://www.invitrogen.com)
- Retention of biotin labeling in bone marrow cells
- Cell surface markers used for analysis were identical to those used for cell cycle analysis with the following exceptions: biotinylated Ryk was replaced with sheep anti-mouse Ryk (AF4649, R&D Systems) with Alexa-Fluor 546-conugated donkey anti-sheep IgG acting as a secondary antibody (Invitrogen, Carlsbad, CA, http://www.invitrogen.com)
- APC-conjugated streptavidin (eBioscience) was used to detect biotin-labeled cells
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