Establishment of TRAIL-Resistant Cell Lines

In order to secure stable TRAIL-resistant cells, parental LS-LiM6 or DLD1 cells were treated with 100 ng/ml human recombinant TRAIL (R&D Systems, Danvers, MA, #375-TEC) for 24 h. The apoptotic cells were then removed and the surviving cells were propagated in the presence of 10 ng/ml TRAIL for a total of five cycles resulting in stable TRAIL-resistant cell lines LiM6-TR and DLD1-TR. The sensitivity of TRAIL-resistant cell lines to TRAIL was periodically examined by PI (Sigma, St. Louis, MO) staining and flow cytometry, and never exceeded 5% cell death as described before.^{10 (link)}

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Ilmer M., Mazurek N., Byrd J.C., Ramirez K., Hafley M., Alt E., Vykoukal J, & Bresalier R.S. (2016). Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics. Cell Death & Disease, 7(8), e2337-.

Publication 2016

human recombinant TRAIL

Apoptotic Cell death Cell lines Flow cytometry Human Parental Sensitivity Trail

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center, Tulane University

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Variable analysis

independent variables

Treatment with 100 ng/ml human recombinant TRAIL for 24 h
Propagation in the presence of 10 ng/ml TRAIL for a total of five cycles

dependent variables

Sensitivity of TRAIL-resistant cell lines to TRAIL, measured by PI staining and flow cytometry

control variables

Parental LS-LiM6 or DLD1 cells

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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