Phospholipid and Lipid Analysis by LC-MS and GC-MS

Analysis of phospholipids by liquid chromatography coupled to mass spectrometry was carried out as described elsewhere (34 (link)–41 (link)), using an Agilent 1260 Infinity high-performance liquid chromatograph equipped with an Agilent G1311C quaternary pump and an Agilent G1329B Autosampler, coupled to an API2000 triple quadrupole mass spectrometer (Applied Biosystems). Phospholipid molecular species were identified by comparison with previously published data (34 (link)–41 (link)). Lipid analysis by gas chromatography coupled to mass spectrometry was carried out exactly as described elsewhere (59 (link)–63 (link)), using an Agilent 7890A gas chromatograph coupled to an Agilent 5975C mass selective detector operated in electron impact mode, equipped with an Agilent 7693 Autosampler and an Agilent DB23 column (60 m length × 0.25 mm internal diameter × 0.15 µm film thickness).

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Rubio J.M., Astudillo A.M., Casas J., Balboa M.A, & Balsinde J. (2018). Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens. Frontiers in Immunology, 9, 1723.

Publication 2018

G1311C quaternary pump G1329B Autosampler API2000 triple quadrupole mass spectrometer 7693 Autosampler DB23 column

Electron Gas chromatograph High performance liquid chromatograph Lipid Liquid chromatography Mass spectrometry Phospholipid

Corresponding Organization : Instituto de Biomedicina y Genética Molecular de Valladolid

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Variable analysis

independent variables

Phospholipid analysis by liquid chromatography coupled to mass spectrometry
Lipid analysis by gas chromatography coupled to mass spectrometry

dependent variables

Phospholipid molecular species
Lipid composition

control variables

Agilent 1260 Infinity high-performance liquid chromatograph equipped with an Agilent G1311C quaternary pump and an Agilent G1329B Autosampler, coupled to an API2000 triple quadrupole mass spectrometer (Applied Biosystems)
Agilent 7890A gas chromatograph coupled to an Agilent 5975C mass selective detector operated in electron impact mode, equipped with an Agilent 7693 Autosampler and an Agilent DB23 column (60 m length × 0.25 mm internal diameter × 0.15 µm film thickness)

positive controls

Comparison with previously published data (34 (link)–41 (link))

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