Antibodies against WHSC1 (1:1000, 65127S; Cell Signaling Technology, Beverly, MA, USA), mTOR (1:1000, 2983T; Cell Signaling Technology, Beverly, MA, USA), P70S6K (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA), S6 (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA), p-mTOR (1:1000, 5536T; Cell Signaling Technology, Beverly, MA, USA), p-P70S6K (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA), p-S6 (1:1000, 4858T; Cell Signaling Technology, Beverly, MA, USA), OXCT1 (1:1000, 12,175-1-AP; Proteintech, Wuhan, Hubei, China), RUVBL2 (1:1000, 10,195-1-AP; Proteintech, Wuhan, Hubei, China), TXNRD1 (1:1000, 11,117-1-AP; Proteintech, Wuhan, Hubei, China), HSD17B4 (1:1000, 15,116-1-AP; Proteintech, Wuhan, Hubei, China), PKM (1:1000, 10,078-2-AP; Proteintech, Wuhan, Hubei, China), EIF3A (1:1000, 26,178-1-AP; Proteintech, Wuhan, Hubei, China), TPM4 (1:1000, 13,741-1-AP; Proteintech, Wuhan, Hubei, China), and P4HB (1:1000, ab2792; Abcam, Cambridge, UK) were used in this study. Western blotting was performed as previously described.17 (link)
Protocol detail
Western Blotting of Cellular Proteins
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Corresponding Organization :
Other organizations : Nanjing Medical University, The First People’s Hospital of Lianyungang, Xian Yang Central Hospital
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Variable analysis
independent variables
- Antibodies against WHSC1 (1:1000, 65127S; Cell Signaling Technology, Beverly, MA, USA)
- MTOR (1:1000, 2983T; Cell Signaling Technology, Beverly, MA, USA)
- P70S6K (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA)
- S6 (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA)
- P-mTOR (1:1000, 5536T; Cell Signaling Technology, Beverly, MA, USA)
- P-P70S6K (1:1000, 9204S; Cell Signaling Technology, Beverly, MA, USA)
- P-S6 (1:1000, 4858T; Cell Signaling Technology, Beverly, MA, USA)
- OXCT1 (1:1000, 12,175-1-AP; Proteintech, Wuhan, Hubei, China)
- RUVBL2 (1:1000, 10,195-1-AP; Proteintech, Wuhan, Hubei, China)
- TXNRD1 (1:1000, 11,117-1-AP; Proteintech, Wuhan, Hubei, China)
- HSD17B4 (1:1000, 15,116-1-AP; Proteintech, Wuhan, Hubei, China)
- PKM (1:1000, 10,078-2-AP; Proteintech, Wuhan, Hubei, China)
- EIF3A (1:1000, 26,178-1-AP; Proteintech, Wuhan, Hubei, China)
- TPM4 (1:1000, 13,741-1-AP; Proteintech, Wuhan, Hubei, China)
- P4HB (1:1000, ab2792; Abcam, Cambridge, UK)
- Not explicitly mentioned
- Not explicitly mentioned
- Not mentioned
- Not mentioned
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