Synthesis of Modified Nucleotides and Oligonucleotides

The chemical synthesis of base-modified ribo- and deoxyribonucleoside triphosphates (rZTP and dZTP, respectively, Supplementary Figure S1), and C⁷G-modified oligonucleotides (Supplementary Table S1), as well as preparation of mutant T7 RNAP variants are described in detail in the Supplementary Data section.

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Yang H., Eremeeva E., Abramov M., Jacquemyn M., Groaz E., Daelemans D, & Herdewijn P. (2023). CRISPR-Cas9 recognition of enzymatically synthesized base-modified nucleic acids. Nucleic Acids Research, 51(4), 1501-1511.

Publication 2023

Deoxyribonucleoside Dztp Oligonucleotides Triphosphates

Corresponding Organization : KU Leuven

Other organizations : Queensland University of Technology, University of Padua

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Variable analysis

independent variables

Chemical synthesis of base-modified ribo- and deoxyribonucleoside triphosphates (rZTP and dZTP, respectively)
Preparation of mutant T7 RNAP variants

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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