Luciferase Expression Optimization in E. coli

Luciferase synthesis from Luciferase T7 Control DNA plasmid (L482B, Promega) was examined using E. coli T7 S30 Extract System (L1130, Promega). The complete protocol for reaction mixture and final solution preparation was followed as described elsewhere38 (link). Peptide concentration of 2, 10, 30, 60 and 100 μM were used for our analysis. Kanamycin (500 μg/mL) and RNase free distilled water was taken as a positive and negative control, respectively. Luciferase Assay Reagent (E1500, Promega) was used to monitor the luciferase luminescence and the samples were measured using a spectrophotometer (Powerscan HT, Biotek Instruments, USA).