The following commercial antibodies were used in this study: anti‐Nup62 (rabbit; Invitrogen, PA5‐21882), anti‐CKAP4/Climp63 (rabbit; Proteintech, 16686‐1‐AP; Fig 2 ), anti‐Calnexin (rabbit; Abcam, ab22595), anti‐Climp63 (mouse; Enzo lifesciences, ALX‐804‐604; Figs 5 and EV2 ), anti‐CREST (human; ImmunoVision, HCT‐0100), anti‐phospho‐Histone H3(S10) (rabbit; Cell Signaling Technology, 9701S), anti‐RTN4/NOGO (rabbit; Proteintech, 10950‐1‐AP), and anti‐POM121 (rabbit; ThermoFisher Scientific, PA5‐85161). Rabbit antibodies against GP210, Nup160, Nup133, Nup107, Nup96, Nup53 and Nup214 have been previously described (Mansfeld et al, 2006 (link); Laurell et al, 2011 (link); Linder et al, 2017 (link)).
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Antibodies Used in Nuclear Pore Complex Study
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Corresponding Organization :
Other organizations : ETH Zurich, Life Science Zurich, Institute of Cancer Research
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Variable analysis
independent variables
- Anti-Nup62 (rabbit; Invitrogen, PA5-21882)
- Anti-CKAP4/Climp63 (rabbit; Proteintech, 16686-1-AP)
- Anti-Calnexin (rabbit; Abcam, ab22595)
- Anti-Climp63 (mouse; Enzo lifesciences, ALX-804-604)
- Anti-CREST (human; ImmunoVision, HCT-0100)
- Anti-phospho-Histone H3(S10) (rabbit; Cell Signaling Technology, 9701S)
- Anti-RTN4/NOGO (rabbit; Proteintech, 10950-1-AP)
- Anti-POM121 (rabbit; ThermoFisher Scientific, PA5-85161)
- Anti-GP210, anti-Nup160, anti-Nup133, anti-Nup107, anti-Nup96, anti-Nup53 and anti-Nup214 (rabbit; previously described)
- Not explicitly mentioned
- Not explicitly mentioned
- Positive controls: None specified
- Negative controls: None specified
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