Placental RNA Extraction Protocol

Were obtained from a representative number of women belonging to each of the pregnant groups, either during cesarean sections or immediately after delivery. For villous RNA studies, dissections of the chorionic plate were collected from three distinct locations on the placental surface as recommended (Roberts et al., 2019 (link)), submerged, and stored in RNA Later Solution (ThermoFisher Scientific). RNAs were isolated as previously described (Aharon et al., 2005 (link)), using Tri-reagent (Sigma-Aldrich Israel LTD) following a standard procedure.

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Aharon A., Rebibo-Sabbah A., Ahmad R.S., Dangot A., Bar-Lev T.H., Brenner B., Cohen A.H., David C.B., Weiner Z, & Solt I. (2023). Associations of maternal and placental extracellular vesicle miRNA with preeclampsia. Frontiers in Cell and Developmental Biology, 11, 1080419.

Publication 2023

RNA Later Solution

Cesarean sections Chorionic Delivery Dissections Placental Rnas Women

Corresponding Organization : Tel Aviv University

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Variable analysis

independent variables

Pregnant groups

dependent variables

Villous RNA studies

control variables

Dissections of the chorionic plate were collected from three distinct locations on the placental surface as recommended (Roberts et al., 2019)
RNAs were isolated as previously described (Aharon et al., 2005), using Tri-reagent (Sigma-Aldrich Israel LTD) following a standard procedure.

controls

No positive or negative controls were explicitly mentioned.

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