THP1 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM l -glutamine, streptomycin (100 μg/ml), and penicillin (100 U/ml) at 37°C in 5% CO2. SS or G0 THP1 cells were prepared by washing with phosphate-buffered saline (PBS) followed by serum starvation at a density of 2 × 105 cells/ml. AraC-treated cells were prepared by treatment with indicated concentrations of AraC for indicated periods of time. THP1 (TIB-202) and monocytes (CRL9855) were obtained from the American Type Culture Collection (ATCC). NOMO1 and MOLM13 were obtained from ATCC and from the Scadden group (1 (link)). Cell lines were tested for mycoplasma (Promega) and authenticated by the ATCC Cell Authentication Testing Service (1 (link)).
Protocol detail
THP1 Cell Culture and Synchronization
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Arac
Cell lines
Cells
Culture cell
Cultured medium
Fetal bovine serum
Glutamine
Monocytes
Mycoplasma
Penicillin
Phosphate
Promega
Saline
Serum
Streptomycin
Corresponding Organization : Harvard University
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Variable analysis
independent variables
- Treatment with indicated concentrations of AraC for indicated periods of time
- Cell response/phenotype
- RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, streptomycin (100 μg/ml), and penicillin (100 U/ml)
- Culturing at 37°C in 5% CO2
- Serum starvation at a density of 2 × 105 cells/ml
- SS or G0 THP1 cells as a control condition
- Positive and negative controls not explicitly mentioned
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