Real-time oxygen consumption rates (OCR) were determined using the Seahorse Extracellular Flux (XF96) analyzer (Seahorse Bioscience, Billerica, MA, USA). H295R cancer cells were seeded into XF96-well cell culture plates (Seahorse Bioscience, Billerica, MA, USA) and incubated overnight at 37 °C in a 5% CO2 humidified atmosphere. After 48 h, cells were treated with curcumin (10, 20 μM) for 16 h. At the end of treatment, cells were processed as previously described [3 (link)]. The obtained OCR values were normalized to the protein content within the individual wells.
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