Eight-week-old male ZDF rats (fa/fa) and their control, male lean rats (−/−), were purchased from Charles River Laboratories International, Inc. (Wilmington, MA, United States). Animals were housed and cared for based on rules acclaimed by the Institutional Animal Care Committee of University of Debrecen in accordance with international regulations (ARVO (Statement for the Use of Animals in Ophthalmic and Vision Research) and the NIH guidelines), and all methodical protocols were approved by this same committee. Animals had free access to water and rodent chow, Purina 5008 diet.
ZDF animals were randomly assigned into two groups: ZDF control group (ZDF) and BGP-15-treated group (BGP). A third group was formed from the lean animals (lean). N = 10 in each groups. From the age of 16 weeks, all animals received oral gavage (through an orogastric feeding tube) once daily throughout the whole study, which lasted for 52 weeks (1 year). The treated group received 10 mg/kg BGP-15 in methyl-cellulose mucilage, while animals in the lean and ZDF groups were gavaged with vehicle only. Dose was based on our former study (Wachal et al., 2020 (link)). BGP-15 was obtained from Sigma-Aldrich-Merck KGaA (Darmstadt, Germany).
ZDF animals were randomly assigned into two groups: ZDF control group (ZDF) and BGP-15-treated group (BGP). A third group was formed from the lean animals (lean). N = 10 in each groups. From the age of 16 weeks, all animals received oral gavage (through an orogastric feeding tube) once daily throughout the whole study, which lasted for 52 weeks (1 year). The treated group received 10 mg/kg BGP-15 in methyl-cellulose mucilage, while animals in the lean and ZDF groups were gavaged with vehicle only. Dose was based on our former study (Wachal et al., 2020 (link)). BGP-15 was obtained from Sigma-Aldrich-Merck KGaA (Darmstadt, Germany).
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