The following fly stocks were used in this study: UAS-H1KD RNAi lines (TH00868.N, TH00825.N, and TH00826.N), a UAS-mof RNAi line (TH00870.N), UAS-dBigH1 RNAi lines (TH11322.N and TH11323.N), UAS-GFP RNAi line (TH00782.N), UAS-GFP (TH10512.N), P[nosP-GAL4::vp16]46 (link), y, hs-FLP; nos<STOP<GAL4::VP16, UASp-GFP/CyO29 (link), P[bamP-GFP]22 (link), Dad-LacZ35 (link), bamΔ86 (ref. 47 (link)), UAS-p35 (ref. 48 (link)), 1824-GAL4 (AB1-GAL4; Bloomington #1824), OK107-GAL4 (w*;;;P{GawB}eyOK107, a gift from Dr Yi Zhong) and c587-Gal4 (Bloomington #25421).
Sense Oligos of the transgenic RNAi lines are:
TH00782.N: 5′-CCCGAAGGTTATGTACAGGAA-3′
TH00825.N: 5′-AAGCAAGAAGGTAGCCTCTAA-3′
TH00826.N: 5′- TAGCGAAAGCGTCAAAGGCAA-3′
TH00868.N: 5′-ACCAGCGACAGTTGAGAAGAA-3′
TH00870.N: 5′-CTCGACCTCAGCGGTGTCCAA-3′
TH11322.N: 5′-CGGCGAAGTGGTGATGGTTAA-3′
TH11323.N: 5′-ATGGTTAAGCGATCCTTTAAA-3′.
All Drosophila stocks were maintained at 25 °C with 60% humidity on standard cornmeal/sugar/agar media unless otherwise specified.
Sense Oligos of the transgenic RNAi lines are:
TH00782.N: 5′-CCCGAAGGTTATGTACAGGAA-3′
TH00825.N: 5′-AAGCAAGAAGGTAGCCTCTAA-3′
TH00826.N: 5′- TAGCGAAAGCGTCAAAGGCAA-3′
TH00868.N: 5′-ACCAGCGACAGTTGAGAAGAA-3′
TH00870.N: 5′-CTCGACCTCAGCGGTGTCCAA-3′
TH11322.N: 5′-CGGCGAAGTGGTGATGGTTAA-3′
TH11323.N: 5′-ATGGTTAAGCGATCCTTTAAA-3′.
All Drosophila stocks were maintained at 25 °C with 60% humidity on standard cornmeal/sugar/agar media unless otherwise specified.
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