Sodium valproate was from Sigma (St. Louis, MO). Neratinib was supplied by Puma Biotechnology Inc. (Los Angeles, CA). Sorafenib tosylate, dasatinib, ruxolitinib, dabrafenib, trametinib and sildenafil were from Selleckchem (Houston TX). Trypsin-EDTA, DMEM, RPMI, penicillin-streptomycin were purchased from GIBCOBRL (GIBCOBRL Life Technologies, Grand Island, NY). All “H” series NSCLC lines were purchased from the ATCC and were not further validated beyond that claimed by ATCC. Cells were re-purchased every ~6 months. ADOR cells were a gift to the Dent lab from a female NSCLC patient. Spiky ovarian cancer cells were kindly provided by Dr. Karen Paz (Champions Oncology, NJ). Commercially available validated short hairpin RNA molecules to knock down RNA / protein levels were from Qiagen (Valencia, CA) (Supplementary Figure 24). Control IgG, anti-PD-1 and anti-CTLA4 endotoxin-free antibodies were purchased from Bio-X cell (West Lebanon, NH). Reagents and performance of experimental procedures were described in refs: 1, 24-28, 45, 46.
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