Bioluminescent Infection Imaging in Mice

Plasmid pBEN276 (54 (link)) was employed to construct a stable reporting system within the chromosome of ExPEC RS218 (25 (link)) for extended trial in vivo. The 5 × 10⁸ CFU bioluminescent RS218 (confirmed by pretest) was intraperitoneally injected into 4-week-old female BALB/c mice (n = 5/group). After 1 h, the animals were intraperitoneally injected with 100 μl of 25 μg gcIFN-20 or PBS. At 4 h post bacterial injection, the mice were anesthetized and transferred to the IVIS (Newton 7.0, Vilber Lourmat, France). The luminescence was tested with an exposure time of 5–15 sec. The imaging system translates the data into false color images that depict the area of strong luminescence with red and mild luminescence with blue. The bioluminescence intensity is proportional to the number of bacteria. The total flux of the target area was quantified by the IVIS software.

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Xiao X., Lu H., Zhu W., Zhang Y., Huo X., Yang C., Xiao S., Zhang Y, & Su J. (2022). A Novel Antimicrobial Peptide Derived from Bony Fish IFN1 Exerts Potent Antimicrobial and Anti-Inflammatory Activity in Mammals. Microbiology Spectrum, 10(2), e02013-21.

Publication 2022

Newton 7.0

Animals Bacteria Balb c mice Chromosome Expec Female Luminescence Mice Plasmid

Corresponding Organization : Qingdao National Laboratory for Marine Science and Technology

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Variable analysis

independent variables

Injection of gcIFN-20 or PBS

dependent variables

Bioluminescence intensity (total flux) of bacterial cells

control variables

Mouse strain (4-week-old female BALB/c mice)
Bacterial strain (ExPEC RS218)
Bacterial cell count (5 × 10^8 CFU)
Route of bacterial administration (intraperitoneal injection)
Time points (1 h after bacterial injection, 4 h after bacterial injection)
Imaging method (IVIS, 5-15 sec exposure time)

controls

Positive control: Injection of 100 μl of 25 μg gcIFN-20
Negative control: Injection of 100 μl of PBS

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