C57BL/6 (B6) mice were purchased from Janvier Laboratories and Foxp3-GFP.KI reporter33 (link) and SMARTA34 (link) mice have been described previously. All mice were housed and bred in SPF or OHB facilities at LASC Zurich, Switzerland. All experiments were reviewed and approved by the cantonal veterinary office of Zurich and were performed in accordance with Swiss legislation.
Lymphocytic choriomeningitis virus (LCMV) WE was propagated on L929 fibroblast cells, Vaccinia Virus (VV) on BSC40 cells. Sex- and age-matched mice, 6–12 weeks of age were infected i.v. with 200 ffu LCMV, or i.p. with 2 × 106 ffu VV. For footpad infections 50 μL of PBS containing 500 ffu of LCMV were injected s.c. into the right hind footpad23 (link). Epicutaneous ear infections were modified from a previous report24 (link) and performed by piercing the ear five times with a bifurcated needle that was dipped into VV stock (7.65 × 108 ffu/mL) in between punches. The contralateral footpads or ears served as a control and were mock infected in an identical manner using sterile PBS. Swelling of the infected site was monitored daily with a spring-loaded caliper.
Lymphocytic choriomeningitis virus (LCMV) WE was propagated on L929 fibroblast cells, Vaccinia Virus (VV) on BSC40 cells. Sex- and age-matched mice, 6–12 weeks of age were infected i.v. with 200 ffu LCMV, or i.p. with 2 × 106 ffu VV. For footpad infections 50 μL of PBS containing 500 ffu of LCMV were injected s.c. into the right hind footpad23 (link). Epicutaneous ear infections were modified from a previous report24 (link) and performed by piercing the ear five times with a bifurcated needle that was dipped into VV stock (7.65 × 108 ffu/mL) in between punches. The contralateral footpads or ears served as a control and were mock infected in an identical manner using sterile PBS. Swelling of the infected site was monitored daily with a spring-loaded caliper.
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