Multivector Genetic Manipulation Protocol
Corresponding Organization : Yale Cancer Center
Other organizations : University of Pittsburgh Medical Center, UPMC Hillman Cancer Center, UConn Health, University of California, Berkeley, University of Colorado Boulder, Stanford University, Palo Alto Institute, Innovative Genomics Institute, The University of Texas Southwestern Medical Center
Variable analysis
- PInducer20 KDM6A WT, MT2 and ΔTPR mutant
- PLV-EF1A-ACE2
- PLX307-DPP4
- Measured outcomes
- Cell confluency (50%) for lentiviral transduction
- Puromycin (5 μg/ml) and G418 (1600 μg/ml) selection 48 hours after transduction
- Positive control: Not specified
- Negative control: Not specified
Annotations
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