All constructs were PCR-amplified from cDNA using Q5 High-Fidelity DNA Polymerase with GC buffer (NEB). pInducer20 KDM6A WT, MT2 and ΔTPR mutant were previously described [32 (link)]. pLV-EF1A-ACE2 was a gift from Dr. Akiko Iwasaki. PLX307-DPP4 was purchased from Addgene (Cat#158451). For lentiviral transduction, cells were transduced at 50% confluency and selected with puromycin (5 μg/ml) or G418 (1600 μg/ml) 48 hours later.
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