LVPO was induced by TAC in 9‐week‐old mice.
22 (link) Mice were subcutaneously pretreated with 1 mg/kg buprenorphine (Bayer, Leverkusen, Germany) and 5 mg/kg carprofen (Cp‐Pharma, Burgdorf, Germany). Anesthesia was induced with 5% isoflurane, sustained with 2% isoflurane, and monitored by toe pinching. Eye care solution was applied to prevent corneal injury and mice were placed in the supine position on a heating pad (37 °C). Mice were orally intubated and mechanically ventilated using a rodent ventilator (Hugo Sachs Elektronik, March‐Hugstetten, Germany). Following horizontal skin incision at the level of the suprasternal notch, the transverse aortic arch was visualized through a median sternotomy. Next, the transverse aortic arch was ligated using a 6–0 silk suture that was tightened around a blunt 25‐G needle, which was placed between the innominate artery and the left common carotid artery. The needle was quickly removed, leaving a discrete region of stenosis. The chest and overlying skin were closed. Animals received metamizole (1A Pharma, Holzkirchen, Germany) in the drinking water (200 mg/kg) for 4 days total, starting the day before surgery. The sham procedure was performed identically, except no suture was placed around the aortic arch.
22 (link) Mice were subcutaneously pretreated with 1 mg/kg buprenorphine (Bayer, Leverkusen, Germany) and 5 mg/kg carprofen (Cp‐Pharma, Burgdorf, Germany). Anesthesia was induced with 5% isoflurane, sustained with 2% isoflurane, and monitored by toe pinching. Eye care solution was applied to prevent corneal injury and mice were placed in the supine position on a heating pad (37 °C). Mice were orally intubated and mechanically ventilated using a rodent ventilator (Hugo Sachs Elektronik, March‐Hugstetten, Germany). Following horizontal skin incision at the level of the suprasternal notch, the transverse aortic arch was visualized through a median sternotomy. Next, the transverse aortic arch was ligated using a 6–0 silk suture that was tightened around a blunt 25‐G needle, which was placed between the innominate artery and the left common carotid artery. The needle was quickly removed, leaving a discrete region of stenosis. The chest and overlying skin were closed. Animals received metamizole (1A Pharma, Holzkirchen, Germany) in the drinking water (200 mg/kg) for 4 days total, starting the day before surgery. The sham procedure was performed identically, except no suture was placed around the aortic arch.
