ELISA for Galectin-3 Quantification

Enzyme‐linked immunosorbent assay (ELISA) plates from Abcam (ab269555) were used to measure the levels of Gal‐3 (detection range 58.8 to 2000 pg/mL) in tissue homogenates, CSF, and serum samples. The protocol was carried out according to the manufacturer's instructions. A Biotek Synergy 2 was used to read the ELISA Gal‐3 assay. All samples were run in duplicate once. Mean inter‐assay Coeficients of Variances (CV) was 6.23. Samples were distributed in the plates according to the clinical group in similar proportions to avoid a bias caused by the plate. The ELISA and the analysis of the raw data were performed by different persons. CSF samples were not diluted. Tissue homogenates were diluted 1:100. Serum samples were diluted 1:20 or 1:50; the correction factor on diluted samples was applied when needed for the comparison. Our kit has been used previously to measure Gal‐3 levels in brain, CSF, and serum samples
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Borrego–Écija S., Pérez‐Millan A., Antonell A., Fort‐Aznar L., Kaya‐Tilki E., León‐Halcón A., Lladó A., Molina‐Porcel L., Balasa M., Juncà‐Parella J., Vitorica J., Venero J.L., Deierborg T., Boza‐Serrano A, & Sánchez‐Valle R. (2023). Galectin‐3 is upregulated in frontotemporal dementia patients with subtype specificity. Alzheimer's & Dementia, 20(3), 1515-1526.

Publication 2023

ab269555 Synergy 2

Corresponding Organization : Instituto de Biomedicina de Sevilla

Other organizations : Biomedical Research Networking Center on Neurodegenerative Diseases, Lund University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of Gal-3 (detection range 58.8 to 2000 pg/mL) in tissue homogenates, CSF, and serum samples

control variables

Samples were distributed in the plates according to the clinical group in similar proportions to avoid a bias caused by the plate
The ELISA and the analysis of the raw data were performed by different persons
CSF samples were not diluted
Tissue homogenates were diluted 1:100
Serum samples were diluted 1:20 or 1:50; the correction factor on diluted samples was applied when needed for the comparison

controls

No positive or negative controls were explicitly mentioned in the input protocol

Annotations

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