Cell samples were washed and stained in flow buffer (PBS, 1% FBS, 0.1% sodium azide) for 20–30 min. Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), PE anti-myc (Clone: 71D10, Cell Signaling Technology), anti-Muc16t was generated and conjugated in house (9 (link),25 (link)), Alexa Fluor 700 anti-mouse CD45 (Clone: 30-F11, BioLegend), and PE anti-CD3 mouse (clone: UCHT1, BioLegend). Data was collected using a Guava easyCyte HT Flow Cytometer (Luminex, Austin, Texas, USA), a LSRFortessa (BD, Franklin Lakes, NJ, USA), or a Cytoflex LX (Beckman Coulter, Pasadena, CA). Data were analyzed using Flowjo v10.4 software (Flowjo, Ashland, OR, USA).
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Flow Cytometry of Mouse Immune Cells
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Corresponding Organization :
Other organizations : Memorial Sloan Kettering Cancer Center, Cornell University, Arsenal Biosciences (United States), Tri-Institutional PhD Program in Chemical Biology
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Variable analysis
independent variables
- Cell samples were washed and stained in flow buffer (PBS, 1% FBS, 0.1% sodium azide) for 20–30 min
- Data was collected using a Guava easyCyte HT Flow Cytometer (Luminex, Austin, Texas, USA), a LSRFortessa (BD, Franklin Lakes, NJ, USA), or a Cytoflex LX (Beckman Coulter, Pasadena, CA)
- Cell samples were washed and stained in flow buffer (PBS, 1% FBS, 0.1% sodium azide) for 20–30 min
- APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences)
- Alexa Fluor 700 anti-mouse CD45 (Clone: 30-F11, BioLegend)
- PE anti-CD3 mouse (clone: UCHT1, BioLegend)
- None specified
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