Flies were raised on standard lab food (corn meal/agar medium). For conditional expression using tubGal80ts, flies were grown at 18 °C until eclosion, maintained at 18 °C for an additional 2d, and then shifted to 29 °C to induce expression. The concentration of chemicals mixed in the fly food was based on previously published reports21 (link),23 (link). The Super CitriMax Garcinia Cambogia extract (Swanson) contains 60% hydroxycitric acid and was purchased from Amazon. Fly food was melted and mixed with a final concentration of 30 mg/ml Garcinia Cambogia extract23 (link). For NaOx feeding, a final concentration of 5 mg/ml sodium oxalate was mixed into melted fly food23 (link). The high purine food contains 20 mM adenine (Sigma-Aldrich, # A8626) and 20 mM guanine (Sigma-Aldrich, # G11950)21 (link).
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