Fluorescence images were acquired by confocal fluorescence microscope unit (AX, Nikon) equipped with a 20× dry objective lens (Plan Apo VC 20×), LASER unit (LUA‐S4), detector unit (DUX‐ST), and a motorized stage. The assay was performed using a solution containing sTM[6 (link)
] (10 µM) as the internal standard, and the focus was adjusted using its fluorescence. Images were acquired in tile scan mode with perfect focus. The excitation wavelength and emission filters used were DAPI (Ex. = 405 nm, Em. = 429–474 nm), FITC (Ex. = 488 nm, Em. = 500–550 nm) and Cy3 (Ex. = 561 nm, Em. = 570–616 nm), respectively. Images were taken as sequential acquisition mode.
] (10 µM) as the internal standard, and the focus was adjusted using its fluorescence. Images were acquired in tile scan mode with perfect focus. The excitation wavelength and emission filters used were DAPI (Ex. = 405 nm, Em. = 429–474 nm), FITC (Ex. = 488 nm, Em. = 500–550 nm) and Cy3 (Ex. = 561 nm, Em. = 570–616 nm), respectively. Images were taken as sequential acquisition mode.
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