Western blot and IHC staining followed established protocols (Yan et al. 2021 (link)). IHC scoring utilized image pro plus software, analyzing images to determine IOD/Area or IOD Sum values. Antibodies used included LOXL1 (1:1000, PA5-79,609, Invitrogen), β-tubulin (1:1000, sc-166729, Santa Cruz) for western blotting, and LOXL1 (1:500), CD8 (ZA-0508, ZSGB-Bio) for IHC.
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