Immunocytochemistry Imaging and Cell Viability

Immunocytochemistry employed standard techniques [32 (link), 33 (link)]. Secondary-antibody-only controls were performed by omitting primary antibodies during incubation (see Supplementary Data). Fluorescent imaging was performed on a Zeiss UV-LSM 510 META and a Nikon A1 confocal microscope. Sequential scanning of channels was performed to prevent false-positive co-localization. Images were quantified using ImageJ. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetra-zolium bromide (MTT) Cell Proliferation Assay Kit [34 (link)].

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Matsuoka A.J., Morrissey Z.D., Zhang C., Homma K., Belmadani A., Miller C.A., Chadly D.M., Kobayashi S., Edelbrock A.N., Tanaka‐Matakatsu M., Whitlon D.S., Lyass L., McGuire T.L., Stupp S.I, & Kessler J.A. (2016). Directed Differentiation of Human Embryonic Stem Cells Toward Placode‐Derived Spiral Ganglion‐Like Sensory Neurons. Stem Cells Translational Medicine, 6(3), 923-936.

Publication 2016

UV-LSM 510 META A1 confocal microscope

Antibodies Antibody Assay Bromide Cell proliferation Cell viability Confocal microscope Diphenyl Immunocytochemistry Tetra

Corresponding Organization : Knowles (United States)

Other organizations : Molecular Biology Consortium, Northwestern University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cell viability

control variables

Standard immunocytochemistry techniques [32, 33]
Sequential scanning of channels to prevent false-positive co-localization
Fluorescent imaging performed on Zeiss UV-LSM 510 META and Nikon A1 confocal microscope
Image quantification using ImageJ

controls

Secondary-antibody-only controls were performed by omitting primary antibodies during incubation

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