Histological analysis of asthma model

Histological experiments were conceived and carried out as previously described (Shi et al., 2020 (link); Li et al., 2023 (link); Peng et al., 2023 (link)). Briefly, tracheal and left lung samples were isolated from experimental groups (control, asthma, dexamethasone and vandetanib). Then the isolated specimens were fixed in 4% paraformaldehyde (PFA) for 12 h at room temperature. Standard histological protocols were employed by Servicebio (Wuhan, China) to perform routine staining experiments such as hematoxylin and eosin (H&E) staining and periodic acid-Schiff (PAS) staining. The bright-field photographs of stained sections were labeled and analyzed. The PAS-positive cells in lung were counted and analyzed by using Fuji ImageJ.

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Zeng X., Xue L., Li W., Zhao P., Chen W., Wang W, & Shen J. (2024). Vandetanib as a prospective anti-inflammatory and anti-contractile agent in asthma. Frontiers in Pharmacology, 15, 1345070.

Publication 2024

ImageJ

Corresponding Organization :

Other organizations : Minzu University of China

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Variable analysis

independent variables

Experimental groups (control, asthma, dexamethasone and vandetanib)

dependent variables

Tracheal and left lung samples
PAS-positive cells in lung

control variables

Tracheal and left lung samples were isolated from experimental groups
Isolated specimens were fixed in 4% paraformaldehyde (PFA) for 12 h at room temperature
Standard histological protocols were employed by Servicebio (Wuhan, China) to perform routine staining experiments such as hematoxylin and eosin (H&E) staining and periodic acid-Schiff (PAS) staining

controls

Control group

Annotations

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