Quantifying Lung Inflammation and Apoptosis

Lung MPO activity, a marker for neutrophil influx, was measured according to the protocols described previously ^{[15 (link),16 (link),17 (link),18 ,19 (link)]}. Active Caspase3 activity in lung tissue homogenate was quantified via established protocols ^{[15 (link),16 (link)]}. Protein concentration was assessed using the Bradford dye-binding procedure (Bio-Rad, Hercules, CA).

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Tang L., Bai J., Chung C.S., Lomas–Neira J., Chen Y., Huang X, & Ayala A. (2015). PD-L1 regulates the Tregs’ capacity to repress shock/sepsis induced indirect lung injury (iALI) by recruiting phosphatase SHP-1. Shock (Augusta, Ga.), 43(1), 47-54.

Publication 2014

Bradford dye-binding procedure

Caspase3 Lung Neutrophil Protein Tissue

Corresponding Organization : Shanghai East Hospital

Other organizations : Rhode Island Hospital, Brown University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Lung MPO activity, a marker for neutrophil influx
Active Caspase3 activity in lung tissue homogenate
Protein concentration

control variables

None explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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