Drosophila Protein Starvation Assay

Flies were cultured in standard Bloomington medium at 25 °C. For protein starvation experiments, newly eclosed adult flies were transferred within 24 hours (day 0) onto either standard food (fed) or 16% sucrose/0.7% agar (starved) at a density of 20–40 flies per vial. Flies were transferred to fresh vials every three days. The following fly stocks were used: c587-gal419 (link), nos-gal448 (link). UAS-spict-mRFP, spict41 (link) were generous gifts of Cahir O’Kane18 (link). UASt-H1-YFP was a kind gift of Alexei Tulin49 (link), tj-gal4 was obtained from Xin Chen35 (link). UAS-grim was obtained from Margaret T. Fuller50 (link). UAS-mCD4-tdTomato, UAS-spict-RNAi^GLC01402, UAS-spict-RNAi^HMS01647, UAS-Rab4-GFP, Rab5-EYFP, Rab7-EYFP, UAS-Rab7-GFP, tub-gal80^ts were obtained from the Bloomington Stock Center. spict-gal4^NP112900 was obtained from the Kyoto Stock Center.

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Chiang A.C., Yang H, & Yamashita Y.M. (2017). spict, a cyst cell-specific gene, regulates starvation-induced spermatogonial cell death in the Drosophila testis. Scientific Reports, 7, 40245.

Publication 2017

UAS-Rab7-GFP tub-gal80ts

Adult Agar Cultured medium Food Gifts Protein Sucrose Tdtomato

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Nutrient condition (standard food vs. 16% sucrose/0.7% agar)

dependent variables

Not explicitly mentioned

control variables

Temperature (25 °C)
Fly density (20-40 flies per vial)
Fly transfer frequency (every three days)

controls

Positive control: Not specified
Negative control: Not specified

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