Genetic Relatedness Analysis of Acinetobacter

Genetic relatedness of the isolates was assessed using semi-automated rep-PCR on the DiversiLab^® System (bioMérieux) after following the manufacturer’s guidelines. Data were analyzed and interpreted using internet-based DiversiLab software (v. 3.4) with the Kullback-Leibler correlation coefficient. Strains presenting a similarity of ≥95% were considered clonally related [13 (link)]. Multilocus sequence typing (MLST) was performed on A. baumannii isolates that were not susceptible to carbapenems following protocols previously described by Bartual et al. 2005 [21 (link)] and Diancourt et al. 2010 [22 (link)] available at the PubMLST website (http://pubmlst.org/acinetobacter, accessed on 30 December 2020).

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Cabral B.G., Brasiliense D.M., Furlaneto I.P., Rodrigues Y.C, & Lima K.V. (2021). Surgical Site Infection Following Caesarean Section by Acinetobacter Species: A Report from a Hyperendemic Setting in the Brazilian Amazon Region. Microorganisms, 9(4), 743.

Publication 2021

DiversiLab® System

Acinetobacter Carbapenems Strains

Corresponding Organization : Instituto Evandro Chagas

Other organizations : Universidade do Estado do Pará

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Variable analysis

independent variables

Genetic relatedness of the isolates assessed using semi-automated rep-PCR on the DiversiLab® System (bioMérieux) after following the manufacturer's guidelines
Multilocus sequence typing (MLST) performed on A. baumannii isolates that were not susceptible to carbapenems following protocols previously described by Bartual et al. 2005 and Diancourt et al. 2010

dependent variables

Strains presenting a similarity of ≥95% were considered clonally related

control variables

Protocols previously described by Bartual et al. 2005 and Diancourt et al. 2010 available at the PubMLST website (http://pubmlst.org/acinetobacter, accessed on 30 December 2020)

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