Polymer PEICT: Cell Migration Assay

The polymer PEICT with an average molecular weight of 46,800 (g/mol) was supplied by SK Chemicals, Gyeonggi-do, Korea [8 (link)]. Chloroform (99%), and trifluoroacetic acid (TFA) (98%) were purchased from Wako, Pure Chemical Industries Ltd., Osaka, Japan. Ethanol (99.8%) was obtained by Merck, Japan. Cell migration on PEICT ENMs was performed using NIH 3T3 mouse fibroblast obtained from ATCC (Manassas, VA), USA [24 (link)]. The 24-well cell culture flasks and Petri-dishes were obtained from SPL Life Sciences (Pocheon, Korea). Ethylenediaminetetraacetic acid (EDTA) was purchased from Merck (KGaA), Germany. Dulbecco’s modified Eagle’s medium (DMEM) was supplied by Nacalai Tesque, Kyoto, Japan. Fetal bovine serum (FBS) and Dulbecco’s phosphate-buffered saline (DPBS) were provided by HyClone (Logan, UT, USA). The Cell Counting Kit-8 (CCK-8) reagent was obtained from Dojindo Laboratories Japan [25 (link)].

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El-Ghazali S., Khatri M., Mehdi M., Kharaghani D., Tamada Y., Katagiri A., Kobayashi S, & Kim I.S. (2021). Fabrication of Poly(Ethylene-glycol 1,4-Cyclohexane Dimethylene-Isosorbide-Terephthalate) Electrospun Nanofiber Mats for Potential Infiltration of Fibroblast Cells. Polymers, 13(8), 1245.

Publication 2021

Chloroform trifluoroacetic acid NIH 3T3 mouse fibroblast Petri-dishes Ethylenediaminetetraacetic acid (EDTA) Dulbecco’s modified Eagle’s medium (DMEM) Cell Counting Kit-8 (CCK-8) reagent

Cell culture Cell migration Chloroform Dishes Eagle Ethanol Ethylenediaminetetraacetic acid Fetal bovine serum Fibroblast Mouse Nih 3t3 Phosphate Polymer Saline Trifluoroacetic acid

Corresponding Organization : Ōtani University

Other organizations : Soochow University, Hiroshima University

Top 5 similar protocols

Variable analysis

independent variables

PEICT polymer with an average molecular weight of 46,800 g/mol

dependent variables

Cell migration on PEICT electrospun nanomembranes (ENMs)

control variables

Chloroform (99%)
Trifluoroacetic acid (TFA) (98%)
Ethanol (99.8%)
NIH 3T3 mouse fibroblast cell line
24-well cell culture flasks and Petri-dishes
Ethylenediaminetetraacetic acid (EDTA)
Dulbecco's modified Eagle's medium (DMEM)
Fetal bovine serum (FBS)
Dulbecco's phosphate-buffered saline (DPBS)
Cell Counting Kit-8 (CCK-8) reagent

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