Immunohistochemical Analysis of ER-α, ER-β, PCNA, and Ki-67

The immunohistochemistry protocol and semi-quantitative analysis were carried out as described in our previous study12 . The following antibodies were used12 23 (link)39 (link): rabbit anti-estrogen receptor-α polyclonal antibody (1:20, SC-542, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-estrogen receptor-β polyclonal antibody (1:50, ab3577, Abcam Biotechnology, Cambridge UK), rabbit anti-proliferating cell nuclear antigen (PCNA) polyclonal antibody (1:15, SC-7907, Santa Cruz Biotechnology), and rabbit anti-ki-67 polyclonal antibody (1:200, SC-7907, Santa Cruz Biotechnology) were used. The Image-Pro Plus 6.0 System image analysis system was used for quantitative analysis.

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Xu Y., Ma X.P., An J.N., Zhang Z.J., Ding J., Qu Y.K., Liu Z.L, & Lin N. (2015). Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice. Scientific Reports, 5, 17436.

Publication 2015

SC-542 ab3577 SC-7907

Anti antibody Antibodies Estrogen receptor Immunohistochemistry Proliferating cell nuclear antigen Rabbit

Corresponding Organization :

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College, Shanghai University of Traditional Chinese Medicine

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Variable analysis

independent variables

Rabbit anti-estrogen receptor-α polyclonal antibody (1:20, SC-542, Santa Cruz Biotechnology, Santa Cruz, CA, USA)
Rabbit anti-estrogen receptor-β polyclonal antibody (1:50, ab3577, Abcam Biotechnology, Cambridge UK)
Rabbit anti-proliferating cell nuclear antigen (PCNA) polyclonal antibody (1:15, SC-7907, Santa Cruz Biotechnology)
Rabbit anti-ki-67 polyclonal antibody (1:200, SC-7907, Santa Cruz Biotechnology)

dependent variables

Quantitative analysis using the Image-Pro Plus 6.0 System image analysis system

control variables

Immunohistochemistry protocol and semi-quantitative analysis were carried out as described in a previous study (no link found)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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