Plasma Carotenoid Analysis by HPLC-DAD
Corresponding Organization : University of Connecticut
Other organizations : Cornell University, The Ohio State University
Variable analysis
- Extraction of lutein and zeaxanthin from plasma
- Quantification of lutein and zeaxanthin in plasma
- Use of Agilent 1260 HPLC-DAD for analysis
- Use of C30 column (4.6 × 250 mm, 3 μm, YMC Inc., Wilmington, DE, USA) for chromatography
- Use of 500 μL of plasma for extraction
- Quantification of carotenoids at 450 nm using authentic external standard curves
- Authentic external standard curves for quantification of lutein and zeaxanthin
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