The rats were randomly divided into the following five groups (n=6): a control group, which received distilled water;
a sham group, which received olive oil as a BPA solvent; and three other groups that were respectively administered 50 mg/kg of BPA, 18 (link)100 mg/kg of RES, 19 (link)and 50 mg/kg of BPA plus 100 mg/kg of RES. Olive oil, BPA, and RES were fed to the animals via gavage for eight weeks. At the end of the experiment,
the rats were subjected to overnight fasting and on the day of euthanasia, they were anesthetized with an intraperitoneal injection of ketamine (Merck, Germany)
at a dose of 100 µg/kg and xylazine (Merck, Germany) at a dose of 5 mg/kg. Liver samples were obtained and fixed in 10% formalin (Merck, Germany)
in preparation for microscopic sections and tissue passaging. Subsequently, the slides were prepared and stained by hematoxylin and eosin (H&E)
(Merck, Germany) staining for isotropic uniform random (IUR) sectioning. The pieces cut with a trocar were finally prepared for the calculation of tissue shrinkage.
a sham group, which received olive oil as a BPA solvent; and three other groups that were respectively administered 50 mg/kg of BPA, 18 (link)100 mg/kg of RES, 19 (link)and 50 mg/kg of BPA plus 100 mg/kg of RES. Olive oil, BPA, and RES were fed to the animals via gavage for eight weeks. At the end of the experiment,
the rats were subjected to overnight fasting and on the day of euthanasia, they were anesthetized with an intraperitoneal injection of ketamine (Merck, Germany)
at a dose of 100 µg/kg and xylazine (Merck, Germany) at a dose of 5 mg/kg. Liver samples were obtained and fixed in 10% formalin (Merck, Germany)
in preparation for microscopic sections and tissue passaging. Subsequently, the slides were prepared and stained by hematoxylin and eosin (H&E)
(Merck, Germany) staining for isotropic uniform random (IUR) sectioning. The pieces cut with a trocar were finally prepared for the calculation of tissue shrinkage.
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