L1 larvae of wild-type (N2) and CF1038 transgenic strains were treated with different concentrations of VVE extract in S-medium for 48 h. After treatment, ROS production was quantified by the DCFH-DA method according to our previous work (15 (link), 17 (link)). The 50-μM DCFH-DA was added into S-medium and incubated in the dark at 20°C for 1 h.
Worm images were examined under a fluorescent microscope (Keyence Deutschland GmbH, Neu-Isenburg, Germany) at least 30 worms per group. The relative fluorescence of the whole body was examined using ImageJ software (National Institutes of Health, Bethesda, MD). The results are presented as mean fluorescence ± SEM.
Worm images were examined under a fluorescent microscope (Keyence Deutschland GmbH, Neu-Isenburg, Germany) at least 30 worms per group. The relative fluorescence of the whole body was examined using ImageJ software (National Institutes of Health, Bethesda, MD). The results are presented as mean fluorescence ± SEM.
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