Characterizing Immune Cell Subsets in Mouse Intestine
Corresponding Organization : Michigan Medicine
Variable analysis
- Genotype of mice (ΔdblGata or WT)
- Frequency and phenotype of CD4+ Th2 cells, ILC2 cells, and MMC9 cells in the lamina propria of the small intestine
- Staining reagents and protocols (phycoerythrin (PE)- conjugated anti-c-Kit, fluorescein isothiocyanate (FITC)-conjugated anti-β7 integrin, Horizon V500-conjugated CD4, APC-Cy-7-conjugated anti-CD3e, allophycocyanin (APC)-conjugated anti-IL-17RB, PE-Cy7-conjugated anti-FcεRIα, biotinylated anti-T1/ST2, and PerCP-Cy5.5-conjugated monoclonal antibodies against lineage (Lin) markers)
- Flow cytometry analysis (BD FACSCanto II instrument and FlowJo software)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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