Blood samples were collected in heparin for measurement of insulin and glucose and in aprotinin/heparin/EDTA for assay of C-peptide [17 (link),46 (link)]. Plasma glucose was determined using Analox GM9 Glucose Analyzer (Analox Instruments, Stourbridge, UK). Insulin (DIAsource, Neuve, Belgium) and C-peptide (Millipore, Billerica, MA, USA) were measured by commercial radioimmunoassay.
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