Multiparametric Flow Cytometry of Immune Cells

The murine splenocytes, TILs and TACs were stained with fluorescein isothiocyanate (FITC)-conjugated CD3 (Biolegend), allophycocyanin (APC)-conjugated CD4 (Biolegend), phycoerythrin (PE)/Cy5.5-conjugated CD8 (Biolegend), PE-conjugated NK1.1 (Biolegend), PE/Cy5.5-conjugated CD19 (Biolegend), APC-conjugated BTLA (CD272) (Biolegend), or PE-conjugated CD223 (eBioscience) for different experiments. Flow cytometric analyses were performed using a BD FACSCalibur flow cytometer (BD Bioscience) with CELLQuest software [23 (link), 24 (link)].

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Chen Y.L., Lin H.W., Chien C.L., Lai Y.L., Sun W.Z., Chen C.A, & Cheng W.F. (2019). BTLA blockade enhances Cancer therapy by inhibiting IL-6/IL-10-induced CD19high B lymphocytes. Journal for Immunotherapy of Cancer, 7, 313.

Publication 2019

FITC)-conjugated CD3 BD FACSCalibur flow cytometer

Allophycocyanin Cd272 Cy5 5 Flow cytometric Isothiocyanate Murine Phycoerythrin Tils

Corresponding Organization :

Other organizations : National Taiwan University

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Variable analysis

independent variables

Staining with fluorescein isothiocyanate (FITC)-conjugated CD3 (Biolegend)
Staining with allophycocyanin (APC)-conjugated CD4 (Biolegend)
Staining with phycoerythrin (PE)/Cy5.5-conjugated CD8 (Biolegend)
Staining with PE-conjugated NK1.1 (Biolegend)
Staining with PE/Cy5.5-conjugated CD19 (Biolegend)
Staining with APC-conjugated BTLA (CD272) (Biolegend)
Staining with PE-conjugated CD223 (eBioscience)

dependent variables

Flow cytometric analyses

control variables

BD FACSCalibur flow cytometer (BD Bioscience) with CELLQuest software

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