Preparation of Stock Solutions for Bioassays

PLU was purchased from Aladdin Reagent Ltd. in Shanghai, China. NIT, CPR, TMP, and CHL were obtained from Sigma-Aldrich, while MEC was sourced from Shanghai Yuanye Biological Co., Ltd. in Shanghai, China. The stock solutions of PLU and antibiotics were prepared in water (MEC) or dimethyl sulfoxide (DMSO) (NIT, CPR, TMP, CHL, and PLU) due to the varying solubility of each compound. The solutions were then passed through 0.22 μm filters and stored in the dark at -20°C. Before being used in assays, the stocks were thawed and diluted to the desired concentrations.

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Bie S., Mo Q., Shi C., Yuan H., Li C., Wu T., Li W, & Yu H. (2024). Interactions of plumbagin with five common antibiotics against Staphylococcus aureus in vitro. PLOS ONE, 19(1), e0297493.

Publication 2024

Corresponding Organization : Tianjin haihe hospital

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Variable analysis

independent variables

PLU purchased from Aladdin Reagent Ltd. in Shanghai, China
NIT, CPR, TMP, and CHL obtained from Sigma-Aldrich
MEC sourced from Shanghai Yuanye Biological Co., Ltd. in Shanghai, China

dependent variables

Not explicitly mentioned

control variables

Stock solutions of PLU and antibiotics prepared in water (MEC) or dimethyl sulfoxide (DMSO) due to varying solubility of each compound
Solutions passed through 0.22 μm filters and stored in the dark at -20°C
Stock solutions thawed and diluted to desired concentrations before being used in assays

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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