Reagent grade 5-chloroisatin, and 2,4-dinitrophenylhydrazine were purchased from Sigma-Aldrich. Ethanol as solvent and concentrated acetic acid were used as purchased.
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Protocol detail
Synthesis of 5-Chloroisatin Phenylhydrazone
Corresponding Organization : Martin Luther University Halle-Wittenberg
Other organizations : University of Maiduguri, The Wistar Institute
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Variable analysis
independent variables
- Amount of 5-chloroisatin
- Amount of 2,4-dinitrophenylhydrazine
- Amount of ethanol
- Amount of concentrated acetic acid
- Not explicitly mentioned
- Reagent grade 5-chloroisatin
- Reagent grade 2,4-dinitrophenylhydrazine
- Ethanol as solvent
- Concentrated acetic acid
- No positive or negative controls were explicitly mentioned.
Annotations
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