Evaluating Cytotoxicity of Ester Derivatives

Trans-RSV, dimethyl sulfoxide (DMSO), TMA, chloroform, 2-mercaptoethanol, and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased directly from Sigma-Aldrich^® (Merck KGaA, Darmstadt, Germany). Penicillin–streptomycin and 0.5% trypsin-EDTA were purchased from Gibco (Thermo Fisher Scientific Inc., Waltham, MA, USA). Human liver cancer cell lines (RM60025), C. asparagiforme (BCRC 80850), and B. longum subsp. longum (BCRC 14664) were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan). ED2 and ED4 were the primary ester derivatives of RBE, and three samples were obtained by this team from the isolation of a previous study [14 (link)].