Immunohistochemical Analysis of CBL-B in Atherosclerosis

Coronary artery specimens were obtained from autopsy from the Department of Pathology of the Amsterdam UMC and immediately fixed in 10% formalin and processed for paraffin embedding. All use of tissue was in agreement with the ‘Code for Proper Secondary Use of Human Tissue in the Netherlands’. CBL-B expression was analysed by immunohistochemistry, as described in the Supplementary material online. Gene expression of CBL-B in human atherosclerosis was examined by microarray-based transcriptional profiling of carotid endarterectomy specimens (BiKE dataset¹³ (link)^,¹⁴ (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Seijkens T.T., Poels K., Meiler S., van Tiel C.M., Kusters P.J., Reiche M., Atzler D., Winkels H., Tjwa M., Poelman H., Slütter B., Kuiper J., Gijbels M., Kuivenhoven J.A., Matic L.P., Paulsson-Berne G., Hedin U., Hansson G.K., Nicolaes G.A., Daemen M.J., Weber C., Gerdes N., de Winther M.P, & Lutgens E. (2018). Deficiency of the T cell regulator Casitas B-cell lymphoma-B aggravates atherosclerosis by inducing CD8+ T cell-mediated macrophage death. European Heart Journal, 40(4), 372-382.

Publication 2018

Atherosclerosis Autopsy Carotid endarterectomy Cbl b Coronary artery Formalin Gene expression Human Immunohistochemistry Microarray Tissue Transcriptional

Corresponding Organization : Ludwig-Maximilians-Universität München

Other organizations : German Centre for Cardiovascular Research, Goethe University Frankfurt, Maastricht University, Centre for Human Drug Research, Leiden University, University Medical Center Groningen, University of Groningen, Karolinska University Hospital, Karolinska Institutet, Düsseldorf University Hospital, Heinrich Heine University Düsseldorf

Top 5 similar protocols

Protocol cited in 3 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

CBL-B expression
Gene expression of CBL-B in human atherosclerosis

control variables

Coronary artery specimens were obtained from autopsy from the Department of Pathology of the Amsterdam UMC
All use of tissue was in agreement with the 'Code for Proper Secondary Use of Human Tissue in the Netherlands'

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!