Evaluating Anti-Inflammatory Potential of Olive Oil Compounds

Bovine Serum Albumin, fluorescein isothiocyanate-dextran (wt 4,000), dimethyl-sulfoxide (DMSO), Bradford reagent, Cell Lytic-M, lipopolysaccharide from Escherichia coli, hydroxytyrosol, tyrosol, NaCl, NaF, K₂HPO₄, KH₂PO₄, MgSO₄x7H₂O, CaCl₂x6H₂O, NaHCO₃, Tween 80 and all solvents of analytical grade were purchased from Sigma Aldrich (Milano, Italy). tyrosol glucuronide, tyrosol sulfate sodium salt, 3′- hydroxytyrosol 3′-glucuronide, hydroxytyrosol 3-sulfate sodium salt, were obtained from LGC standards (Sesto San Giovanni, Italy). The Phosphatase and Protease Inhibitor Cocktail, nitrocellulose membranes, gels and all material for electrophoresis were purchased from ThermoFisher Scientific (Massachusetts, United States). ReagentPack Subculture Reagents with Trypsin/EDTA, TNS (Trypsin Neutralizer solution) and HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid) solutions were obtained from Lonza (Basel, Switzerland). Transwell inserts were obtained from Corning Costar Corp. (New York, N.Y., United States).