Western Blot Analysis of Cell Adhesion Proteins

Mouse anti-E-cadherin, anti-p120^ctn, and anti-δ-catenin clone 30 (to amino acids 85-194) were from BD Biosciences (Palo Alto, CA). Monoclonal anti-GAPDH was from EMD Science. Rabbit, guinea pig, and mouse antibodies against different epitopes of mouse or human δ-catenin (epitopes on mouse 85-194, 292-309, 691-708, 1017-1035; human 434-530, 828-1022) were described previously (4 (link), 5 (link)). Rabbit anti-PARP and anti-PKM2 were from Cell Signaling. Rabbit anti-HIF-1α and anti-Bcl-2 were from Santa Cruz Biotechnology whereas rabbit anti-β-catenin was from Sigma. Rabbit anti-Glut-1 and mouse anti-mitochondrial NADPH dehydrogenase/complex cocktail were from Abcam. All other chemicals were from sigma unless indicated otherwise.

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Nopparat J., Zhang J., Lu J.P., Chen Y.H., Zheng D., Neufer P.D., Fan J., Hong H., Boykin C, & Lu Q. (2014). δ-Catenin, a Wnt/β-Catenin Modulator, Reveals Inducible Mutagenesis Promoting Cancer Cell Survival Adaptation and Metabolic Reprogramming. Oncogene, 34(12), 1542-1552.

Publication 2014

Mouse anti-E-cadherin anti-p120ctn Rabbit anti-PARP anti-PKM2 Rabbit anti-HIF-1α anti-Bcl-2 rabbit anti-β-catenin Rabbit anti-Glut-1

Amino acids Antibodies Bcl 2 Cadherin Catenin Clone Epitopes Gapdh Glut 1 Guinea pig Human Mitochondrial Mouse Nadph dehydrogenase P120ctn Rabbit β catenin

Corresponding Organization :

Other organizations : East Carolina University, Zhejiang University, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

Mouse anti-E-cadherin
Anti-p120^ctn
Anti-δ-catenin clone 30 (to amino acids 85-194)
Rabbit, guinea pig, and mouse antibodies against different epitopes of mouse or human δ-catenin (epitopes on mouse 85-194, 292-309, 691-708, 1017-1035; human 434-530, 828-1022)

dependent variables

Not explicitly mentioned

control variables

Monoclonal anti-GAPDH
Rabbit anti-PARP
Rabbit anti-PKM2
Rabbit anti-HIF-1α
Rabbit anti-Bcl-2
Rabbit anti-β-catenin
Rabbit anti-Glut-1
Mouse anti-mitochondrial NADPH dehydrogenase/complex cocktail

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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