Fasting Metabolite Measurement in Mice

Retro-orbital venous blood was drawn at 1100 h from mice fasted overnight to assess plasma insulin levels (Linco Research), nonesterified fatty acids (NEFA C; Wako), triacylglycerol (Pointe Scientific), and fibroblast growth factor 21 (FGF21; BioVendor) (23 (link)).

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Al-Share Q.Y., DeAngelis A.M., Lester S.G., Bowman T.A., Ramakrishnan S.K., Abdallah S.L., Russo L., Patel P.R., Kaw M.K., Raphael C.K., Kim A.J., Heinrich G., Lee A.D., Kim J.K., Kulkarni R.N., Philbrick W.M, & Najjar S.M. (2015). Forced Hepatic Overexpression of CEACAM1 Curtails Diet-Induced Insulin Resistance. Diabetes, 64(8), 2780-2790.

Publication 2015

NEFA C FGF21

Fgf21 Insulin Mice Nefa Plasma Triacylglycerol Venous blood

Corresponding Organization : University of Toledo

Other organizations : University of Massachusetts Chan Medical School, Harvard University, Joslin Diabetes Center, Yale University

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Fasting overnight

dependent variables

Plasma insulin levels
Nonesterified fatty acids (NEFA C)
Triacylglycerol
Fibroblast growth factor 21 (FGF21)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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