Caspase-3/7 Activity in Kidney Apoptosis

Apoptosis was assessed in whole kidney lysates using the Caspase-Glo 3/7 luminescence assay (Promega) measuring levels of active-caspase 3/7, as previously described(29 (link)). Luminescence was measured using a Turner Biosystems luminometer (Promega). Experiments were performed in 10 independent kidney samples each, from RCALC2 mutant mice and WT littermates. Data was expressed as fold change±SEM for each group. Statistical analyses were performed using two-way ANOVA.

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Gorvin C.M., Ahmad B.N., Stechman M.J., Loh N.Y., Hough T.A., Leo P., Marshall M., Sethi S., Bentley L., Piret S.E., Reed A., Jeyabalan J., Christie P.T., Wells S., Simon M.M., Mallon A., Schulz H., Huebner N., Brown M.A., Cox R.D., Brown S.D, & Thakker R.V. (2018). An N‐Ethyl‐N‐Nitrosourea (ENU)‐Induced Tyr265Stop Mutation of the DNA Polymerase Accessory Subunit Gamma 2 (Polg2) Is Associated With Renal Calcification in Mice. Journal of Bone and Mineral Research, 34(3), 497-507.

Publication 2018

Caspase-Glo 3/7 luminescence assay Turner Biosystems luminometer

Anova Apoptosis Caspase 7 Kidney Luminescence Luminescence assay Mice Promega

Corresponding Organization : University of Oxford

Other organizations : Medical Research Council, Translational Research Institute, Queensland University of Technology, Max Delbrück Center

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Variable analysis

independent variables

RCALC2 mutant mice
WT littermates

dependent variables

Levels of active-caspase 3/7 (measured using Caspase-Glo 3/7 luminescence assay)

control variables

Whole kidney lysates

controls

Positive control: Not specified
Negative control: Not specified

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